Module: 1
Sponsoring Program: CCB
Administrator: Arezou Razavi

STUDY LIST INFORMATION
Course Number: Chem 219
Course Name: Proteomics and Mass Spectrometry
Grading Option: S/U
Course Director: Al Burlingame

MORE COURSE INFORMATION
Dates: April 3 - 21
Campus: MB
Location: BH 212
Schedule: M/Th, 1:00 - 3:00 pm
Maximum Class Size: 12

This course will focus on the practical aspects (i.e. experimental and mass spectral interpretation) involved in the identification of proteins and their covalent modifications. It will cover the fundamental principles of currently important mass spectrometry instrument platforms. It will provide an overview of key scientific problems that are being tackled and solved at the protein level relevant to cell function/dysfunction; the detection and assignment of protein posttranslational modifications; and studies of site-specific dynamics/relative quantitation. It will also cover studies on the architecture of protein complexes and machines.

Methods for sample preparation and mass spectral data acquisition will be discussed together with how our UCSF bioinformatics toolbox (ProteinProspector) may be employed to facilitate the interpretation and presentation of mass spectral information.

Simultaneously, dry laboratory work is required to learn how to carry out the interpretation of complex mixtures of peptides isolated from cells involved in current active research projects. Initially blocks of time will be scheduled for class discussion of the experimental results you obtain.