BMS CCB 219 Workshop on display technologies for recombinant binding proteins (2021)

Module: 2
Sponsoring Program: CCB
Administrator: Julia Molla

STUDY LIST INFORMATION
Course Number: CCB 219
Course Name: Special Topics
Units: 3
Grading Option: S/U
Course Director: Jim Wells

MORE COURSE INFORMATION
Dates: 4/19-5/7
Schedule: 3 x 90 min sessions per week. Tues, Thurs, Fri, 1-2:30p
Maximum Class Size:  20

Course Description:
Eight lectures 50 min total (two lecturers per session; 25 min each); 40 min literature discussion (1-2 papers chosen by lecturers and presented by participants)
Ninth section will be proposals presented by participants broken into 5 groups

  1. Review popular display technologies (phage, yeast, mammalian, Ribosome) (Jim Wells) Antibody expression and characterization from beginning to end (sequence, informatics, affinity, specificity, epitope binning, developability) (Kevin Leung) (No Discussion Session for this intro Session) April 20
  2. Antigen expression and preparation (soluble protein, Fc Fusions, complex systems like cells or even yeast, IVTT, immobilization methods, MHC-peptide) (Kevin Leung, Nick Rettko) April 22
  3. Library creation for naïve binders and affinity optimization (nature inspired, codon restricted, soft vs hard randomization, error-prone PCR, templates and kill sites, Kunkel etc) (Cole Bracken, Jeff Glasgow) April 23
  4. Display Scaffolds (Fabs, VH, other domains and proteins (like DARPins, Ubiquitin) cyclic peptides, bi-cycles, chemically modified libraries) (Cole Bracken, Susanna Elledge) April 27
  5. Selection strategies (standard positive selections, differential selections for specific epitopes, masked selections, functional selections etc) (Jie Zhou, Emily Kang) April 29
  6. Challenging targets (membrane proteins/GPCRs, peptides, specific conformations, PTM’s) (Aashish Manglik, Xin Zhou) April 30
  7. Phage display for enzyme inhibitors and substrates (Protease inhibitors, substrate phage)(Charly Craik , Jie Zhou) May 4
  8. Bi-specifics and hybrid modalities (BiTEs, AbCIDs, bi-paratopic, AbTAC) (Adam Cotton, Jim Wells) May 6
  9. Final Project Presentations: Propose an important biological problem that can be uniquely solved by having recombinant binders. Each of 5 groups presents proposal (15 min each). Propose target(s)/technology and the biology you wish to probe and how you would best find/validate protein binders and use them.  May 7