Biochem 210 High-throughput sequencing, from bench to laptop (2016)

Module: 2
Sponsoring Program: Tetrad
Administrator: Toni Hurley

Course Number: Biochem 210
Course Name: High-throughput sequencing, from bench to laptop
Units: 3
Grading Option: S/U
Course Director: David Weinberg (David Morgan on study list)

Additional Course Director(s): Matthew Thomson
Room Number: GH S-204

Campus: Mission Bay
Schedule: April 18-29;  M-F 1:00-4:00 PM
Prerequisites: None
Maximum Class Size: 15

Course Description: In 2006, the introduction of the Solexa Genome Analyzer enabled scientists to sequence one gigabase of data (that’s 1,000,000,000 bases!) in a single run. Since then, high-throughput sequencing has revolutionized how we do biology research—and many of you are likely to use this technology at some point during your thesis research­. This course will cover the entire process of high-throughput sequencing, from library preparation at the bench through data analyses at the computer. Approximately half of the course time will focus on the molecular biology underlying high-throughput sequencing, including cDNA library generation and sequencing-by-synthesis. The other half will explore how to deal with the massive amounts of resulting data, including mapping reads to the genome and performing gene expression analyses. As a starting point, the course will focus on the core RNA-seq technology. Students will then use this knowledge to understand other RNA-sequencing-based approaches (e.g., ribosome profiling, RNA-modification mapping, and RNA structure probing) and present their findings to the class. The course will consist of a mixture of short lectures, paper discussions, hands-on tutorials, small group presentations, and practical exercises. No prior experience with either molecular biology or computational analysis is required.